1. RFP : 다중 감염성 질환 스크리닝을 위한 멀티채널 진단키트 개발

2. 해당분과 : 4-2-1과제

3. 과제명 : 4분류 질환의 현장 동시 검출용 유전자 분석 기반

4. 연구책임자 : 임채승(고려대학교)

[Abstract]

Acute myocardial infarction (AMI) is one of the most serious diseases affecting human beings. In this study, in order to rapidly detect AMI disease, the authors fabricated a label-free electrochemical biosensor composed of a multi-functional DNA structure on Au nanospike (AuNS) with a fabricated Au micro-gap electrode which was incorporated with a PCB chip in order to detect cardiac troponin I (cTnI). As a bioprobe, the DNA 3 way-junction (3WJ) was introduced, because the DNA 3WJ has three arms for embodying the multi-functionality. Each piece of DNA was assembled to simultaneously form the DNA 3WJ for cTnI detection, signal transduction, and immobilization, respectively. The assembled DNA 3WJ structure was confirmed by Native-TBM PAGE. Moreover, in order to increase the electrochemical signal sensitivity, AuNS was prepared. The Au micro-gap array is fabricated with a printed circuit board (PCB) chip in order to control each micro-gap electrode panel selectively so as to detect low volumes of cTnI. Then, the DNA strucuture on pAuNS-modified electrode was prepared using the layer-by-layer (LbL) assembly method. FE-SEM and AFM were used to investigate the modified-surface morphology. The cyclic voltammetry (CV) was measured to confirm the cTnI binding to DNA 3WJ-modified electrode. cTnI was detected in the HEPES solution and human serum, respectively. The LOD result exhibited 1.0 pM in HEPES solution and 1.0 pM in 20% diluted human serum, respectively. In addition, the selectivity test was carried out with various proteins as the control experiment. The present study showed label-free, simple fabrication, and easy-to-tailor detection elements for cTnI.