1. RFP : SFTS 감염실태 및 전파 방지 연구

2. 해당분과 : 2-1-4 과제

3. 과제명 : 중증열성혈소판감소증바이러스 (SFTSV)에 대한인체감염모델 확립을 위한 최적의 실험동물 발굴 및 이를 이용한 백신/치료물질 유효성 검증모델 구축

4. 연구책임자 : 정혜원(충북대학교)

[Abstract]

Background : Although human-to-human transmission of severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) via direct contact with body fluids has been reported, the role of specific body fluids from SFTSV-infected hosts has not been investigated in detail.

Methods : To demonstrate the virus transmission kinetics in SFTSV-infected hosts, we adapted the ferret infection model and evaluated the virus shedding periods, virus titers, and transmission modes from various specimens of infected ferrets.

Results : Large amounts of infectious SFTSV are shed through nasal discharge, saliva, and urine from SFTSV-infected ferrets. Virus could be detected from 2 dpi and persisted until 12 dpi in these specimens, compared with the relatively short virus-shedding period in sera. Further, transmission studies revealed that SFTSV can be transmitted to close direct and indirect contact naïve animals through various mediums, especially through contact with serum and urine. Further, ferrets contacted with human urine specimens from SFTSV-positive patients were successfully infected with SFTSV, suggesting that urine specimens could be a source of SFTSV infection in humans.

Conclusions : Our results demonstrate that the SFTSV can be shed in various body fluids for more than 12 days and that these specimens could be a source for direct or indirect transmission through close personal contact.

Keywords : body fluids; ferret; indirect transmission; SFTSV; virus shedding.